Involvement of Histone H3 (Ser10) Phosphorylation in Chromosome: INTRODUCTION
The meiotic resumption of oocytes is controlled by protein kinase MPF (maturation-promoting factor or M-phase promoting factor), which is now also known as Cdc2 kinase. Cdc2 kinase is activated around...
View ArticleInvolvement of Histone H3 (Ser10) Phosphorylation in Chromosome: MATERIALS...
Culture of Pig Oocytes Pig ovaries were obtained from prepubertal gilts at a local slaughterhouse. The ovaries were washed once with 0.2% (w/v) cetyltrimethylam-monium bromide and twice with Dulbecco...
View ArticleInvolvement of Histone H3 (Ser10) Phosphorylation in Chromosome: RESULTS
Phosphorylation of Histone H3 Takes Place During Oocyte Maturation Phosphorylated histone H3 at Ser10 was detected in pig maturing oocytes using an antibody against histone H3 with a single...
View ArticleInvolvement of Histone H3 (Ser10) Phosphorylation in Chromosome: DISCUSSION
Pig oocytes undergo GVBD in a similar time course required to activate the Cdc2 kinase. Following the gonadotropic stimulation, pig oocytes start to accumulate cy-clin B1 at 21 h, and Cdc2 kinase...
View ArticleIsoforms of the Mitochondrial Capsule Protein: INTRODUCTION
After exiting the testis, mammalian spermatozoa undergo morphological and biochemical changes as they travel through the epididymis. As a result of these modifications, spermatozoa acquire progressive...
View ArticleIsoforms of the Mitochondrial Capsule Protein: MATERIALS AND METHODS
Animals Care and use of animals conformed to National Institutes of Health guidelines for humane animal care and use in research, and all protocols were approved by the institutional Animal Care and...
View ArticleIsoforms of the Mitochondrial Capsule Protein: RESULTS
Pattern of Capacitation-Dependent Tyrosine-Phosphorylated Polypeptides of Cauda Epididymal Spermatozoa Western blots of capacitated sperm lysates stained with anti-pY20 revealed a spectrum of tyrosine...
View ArticleIsoforms of the Mitochondrial Capsule Protein: DISCUSSION
Protein tyrosine phosphorylation is one of the important biochemical mechanisms regulating sperm capacitation. In the present study, we found the time-dependent increase in protein tyrosine...
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