Culture of Pig Oocytes Pig ovaries were obtained from prepubertal gilts at a local slaughterhouse. The ovaries were washed once with 0.2% (w/v) cetyltrimethylam-monium bromide and twice with Dulbecco phosphate-buffered saline (PBS) containing 0.1% (w/v) polyvinyl alcohol (PbS-PVA; Sigma Chemical Co., St. Louis, MO). Antral follicles 4-6 mm in diameter were dissected in PBS-PVA from […]
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